Fig. 7

HNRNPL promotes the production of circLMO7 in GC. a. RBPmap showed the binding sites between the flanking introns of circLMO7 exons and HNRNPL. b, c. RNA pull-down and RNA immunoprecipitation assays showed that HNRNPL can bind to the flanking introns of circLMO7 exons. d, e. qRT-PCR showed that the wild-type plasmid could significantly increase the expression of circLMO7, while the mutant plasmids could not. f. qRT-PCR showed the expression levels of circLMO7 and pre-mLMO7 after knocking down HNRNPL. g. Immunohistochemistry showed that HNRNPL was highly expressed in GC tissues; scale bar = 100 μm. h. The TCGA database showed that the expression of HNRNPL in GC tissues was significantly higher than that in normal tissues. i, j. qRT-PCR showed that HNRNPL was highly expressed in GC tissues and was positively correlated with circLMO7. All data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001