Fig. 6

Transfection with HDAC3 shRNA in HL60/ADR cells reduces cell proliferation, increases cell apoptosis, induces cell cycle arrest at G0/G1 phase, and suppresses AKT-P21-CDK2 signaling pathways. a CCK-8 assays were used to assess cell proliferation ability. b Cell apoptosis was analyzed after HDAC3 knockdown by flow cytometry using Annexin-V/PI staining. c is a representative of flow cytometry plot for detection of apoptosis. d-e Cell cycles of HL60/ADR cells were analyzed by flow cytometry after PI staining. f is a representative of flow cytometry for cell cycle detection. g Expression levels of HDAC3, AKT, P21, and CDK2 were measured by RT-PCR. h Expression levels of HDAC3, P-AKT, AKT, P21, and CDK2 were measured by Western blot. GAPDH was used as an internal control. Data represents three independent experiments, and results are shown in the format, mean ± S.D. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: P > 0.05)