Fig. 2
CircCDKN2B-AS1 knockdown suppresses the growth and vitality of cervical cancer cells. a The levels of circCDKN2B-AS1 and linear CDKN2B-AS1 expression in SiHa (left panel) and CaSki (right panel) cells after transfection with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA were determined by qRT-PCR (mean ± SEM, n = 3, one-way ANOVA). b The growth curve was determined with CCK-8 assays after transfection with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA. Left panel, SiHa cells; right panel, CaSki cells (mean ± SEM, n = 3, one-way ANOVA). c Apoptosis level after transfection with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA. Left upper panel, SiHa cells; left lower panel, CaSki cells; right panel, the quantification results of early apoptosis (mean ± SEM, nSiHa = 4, nCaSki = 3, one-way ANOVA). d Left panel: representative images of the migration capability of SiHa and CaSki cells transfected with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA. Right panel: quantification of migration assays (mean ± SEM, n = 9, one-way ANOVA). e Left panel: representative images of the invasion capability of SiHa and CaSki cells transfected with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA. Right panel: quantification of invasion assays (mean ± SEM, n = 9, one-way ANOVA). f Left panel: representative images of wound healing assays of SiHa and CaSki cells transfected with two circCDKN2B-AS1 backsplicing-specific siRNAs or a negative control siRNA. Right panel: quantification of wound healing assays (mean ± SEM, n = 3, one-way ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001