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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: CircCDKN2B-AS1 interacts with IMP3 to stabilize hexokinase 2 mRNA and facilitate cervical squamous cell carcinoma aerobic glycolysis progression

Fig. 6

CircCDKN2B-AS1 regulates the expression of HK2 mRNA by recruiting IMP3 in vivo. a Left: the volume of subcutaneous tumors in nude mice with the sh-circCDKN2B-AS1 or negative control (mean ± SEM, n = 10, unpaired Student’s t-test); middle: images of mice sacrificed at week 5 after injection (n = 6); right upper: macro images of subcutaneous tumors; right lower: representative images of HE staining of subcutaneous tumors. b The levels of circCDKN2B-AS1(left) and HK2 mRNA (middle) expression in transplanted tumor tissues (mean ± SEM, n = 6, unpaired Student’s t-test). Right: statistical analyses of the association between circCDKN2B-AS1 and HK2 mRNA (n = 12, Pearson’s correlation coefficient). c Representative immunohistochemical staining images (upper) and quantification of HK2 protein expression (lower) in transplanted tumors (mean ± SEM, n = 6). d Upper: representative images of 18FDG-microPET/CT scans of transplanted tumors in nude mice. Lower: quantification of the average SUV of transplanted tumors (mean ± SEM, n = 4 each group, unpaired Student’s t-test). e Left: representative images of yolk sacs in zebrafish with circCDKN2B-AS1-overexpressing SiHa cells(n = 20) or negative controls (n = 14). Right: quantification of the relative area of fluorophores in zebrafish yolk sacs (unpaired Student’s t-test). f The volume of subcutaneous tumors in nude mice with negative control plus si-NC, circCDKN2B-AS1 overexpression plus si-NC, circCDKN2B-AS1 overexpression plus si-HK2, and negative control plus si-HK2 (mean ± SEM, n = 5, unpaired Student’s t-test). Macro images (middle) and weights (right) of transplanted tumors when mice were sacrificed at week 4 after tumor cell injection (n = 5, one-way ANOVA). g PA (upper) and LA (lower) contents of transplanted tumors (n = 5, one-way ANOVA, PA: μg/g, LA: μmol/g). h The levels of circCDKN2B-AS1 (left upper) and HK2 mRNA expression (left lower) in transplanted tumor tissues (mean ± SEM, n = 5, unpaired Student’s t-test). Right: the association between circCDKN2B-AS1 and HK2 mRNA (n = 10, Pearson’s correlation coefficient). i qRT-PCR following RIP assays showing the 3’UTR of HK2 mRNA recruited by the IMP3 protein in lysates of transplanted tumor tissues (min to max, n = 5, Kruskal-Wallis Test). *P < 0.05, **P < 0.01, ***P < 0.001

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