Fig. 3

sTRAIL treatment triggered an efficient apoptotic cascade in siCHKA OC cells. a. Live 40× microscopy images of siCHKA OC cell lines untreated or treated with sTRAIL. Scaled bar = 100 μm. b. Representative flow cytometry analysis showing Annexin V/To-PRO-III staining of siCHKA and control OC cells following sTRAIL treatment. Q4: healthy cells, Q3: early apoptotic cells, Q2: late apoptotic cells, Q1: necrotic cells/debris (for each quadrant, the cell percentage on total count events is reported). c. Left panel: Representative Western blotting for PARP and Caspase-3 expression in siCHKA and siNT cell lines after sTRAIL treatment. Arrows indicate the cleaved forms of the proteins. Vinculin was used as loading control. Right panel: densitometric analysis of cleaved PARP and Caspase-3 proteins in siCHKA sTRAIL-treated cells versus control (untreated) cells. d. Percentage of TRAIL-mediated cell growth inhibition (16 h sTRAIL treatment) of OC cells siNT (black bars) and siCHKA (white bars) alone or in presence of pan-caspases inhibitor (red bars), Caspase-9 inhibitor (grey bars), Caspase-8 inhibitor (blue bars). Statistical differences are reported