Fig. 7
Two splice variants of MIR31HG are required for BLCA tumorigenesis with cell specificity. (A-C) Cell viability was quantified using the MTS assay. MIR31HGΔE1 knockdown showed a significant decrease in absorbance in T24 (p = 0.0018, a and UMUC3 (p = 0.0029, b cells, and MIR31HGΔE3 knockdown showed a significant decrease in absorbance in SCaBER (p = 0.0346, c cells after 72 h. d Colony formation was detected by measuring cell staining after 7 days. T24, UMUC3, and SCaBER cells with MIR31HGΔE1 and MIR31HGΔE3 knockdown by specific siRNA showed fewer cell colonies with cell specificity. e Staining intensity percentage of colony formation assay was analyzed by ImageJ sofware. T24 and UMUC3 cells with MIR31HGΔE1 knockdown and SCaBER cells with MIR31HGΔE3 knockdown showed a significant decrease in staining intensity compared to the control group. f Wound healing assay was measured using cell culture inserts. T24, UMUC3, and SCaBER cells with MIR31HGΔE1 and MIR31HGΔE3 knockdown by specific siRNA showed a larger open wound area compared with cell specificity after 12 h. g Difference in open wound area was quantified by calculating the percentage of change in open wound area after 12 h. T24 and UMUC3 cells showed fewer changes in the open wound area, in both MIR31HGΔE1 and MIR31HGΔE3 knockdown groups. SCaBER cells showed fewer changes in the open wound area in the MIR31HGΔE3 knockdown group