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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Indirect cholinergic activation slows down pancreatic cancer growth and tumor-associated inflammation

Fig. 1

Acetylcholinesterase (AChE) expression in normal pancreas (NP), pancreatic intraepithelial neoplasia (PanIN) and pancreatic cancer (ductal adenocarcinoma/PCa). a-c Representative immunohistochemical (IHC) photomicrographs of AChE-immunostained normal human pancreas. d-f Representative images showing IHC staining of AChE in PanIN Grade 3 lesions. g-i Double-immunofluorescence staining for CK-19, AChE and DAPI in pancreatic cancer. j-l Representative images of double-immunofluorescence staining for CK-19, AChE and DAPI at sites of (peri)neural invasion in human pancreatic cancer specimens. m Immunoblot analysis of AChE (110 kDa & 76 kDa) in mouse brain (mBrain) and mouse dorsal root ganglia (mDRG) mouse as control tissue, in human pancreatic cancer (PCC)-lines (T3M-4, SU.86.86 and Panc-1) as well as colon carcinoma cell lines SW620 and DLD-1 and the glioblastoma cell line LN229. n Densitometry graph depicts the quantification of AChE signal relative to alpha-Tubulin content in mDRG, SU.86.86, T3M-4 and Panc1 (n = 3 biological replicates), (SU.86.86 p = 0.0112; T3M-4 p = 0.0018; Panc1 p = 0.0074). Results depicted as Mean ± SD. *p < 0.05, **p < 0.01

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