Table 1 Primary antibodies. IF: immunofluorescence, WB: Western blot, IHC: immunohistochemistry
Antibody | Clone | Species | Company | Concentration |
|---|---|---|---|---|
p44/42 MAPK (Erk1/2) | 137F5 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | D13.14.4E XP® | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Src | 36D10 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Phospho-Src (Ser17) | D7F2Q | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
AMPKα | D5A2 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Phospho-AMPKα (Thr172) | 40H9 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
p38α MAPK | 7D6 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Phospho-p38 MAPK (Thr180/Tyr182) | D3F9 XP® | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
Anti-Acetylcholine-esterase (AChE) | Rabbit pAb | Prestige Antibodies®, Sigma-Aldrich, Taufkirchen. Germany | 1:200 (IF) 1:1000 (WB) 1:400 (IHC) | |
Anti-alpha-Tubulin | Ab11034 | Rabbit | Abcam, Cambridge, UK | 1:10.000 |
F4/80 | Ab6640 | Rat | Abcam, Cambridge, UK | 1:75 |
CD45 | Ab10558 | Rabbit | Abcam, Cambridge, UK | 1:100 |
ChAT | Polyclonal | Rabbit | Kindly provided by Prof. M. Schemann, TU Munich | 1:1000 |