Fig. 6

PIK3R3 is directly targeted by miR-193a-5p and indirectly regulated by circRNA_0000392. a The relative mRNA expression of EPHA2, PIK3R3, EGFR, USP22 and DDX58 after transfection with the miR-193a-5p inhibitor was detected in SW620 cells by qRT-PCR. b Schematic illustration of PIK3R3 3’UTR wild-type (WT) or 3’UTR mutant (MUT) luciferase reporter vectors and the predicted binding sites to miR-193a-5p. c The relative luciferase activities were detected in 293 T cells after cotransfection with the PIK3R3 3’UTR wild-type (WT) or 3’UTR mutant (MUT) luciferase reporter vectors with the miR-193a-5p mimics. d Relative PIK3R3 mRNA expression after transfection with the miR-193a-5p mimics or inhibitor was detected in cells by qRT-PCR. e The relative PIK3R3 protein level after transfection with the miR-193a-5p mimics was detected in cells by western blot. f Relative expression of PIK3R3 in 40 pairs of CRC and ANT tissues measured by qRT-PCR. g Relative PIK3R3 mRNA expression after transfection with circRNA_0000392 siRNA was detected by qRT-PCR. h Representative IHC staining images of low and high PIK3R3 expression in patient CRC tissue samples. Scale bar = 20 μm. i The correlation between circRNA_0000392 and PIK3R3 protein expression in CRC tissues was analyzed based on Spearman correlation coefficients. Data represent the mean ± SD. * P < 0.05, ** P < 0.01