Fig. 7
Identification of bacterial markers discriminating BRAF status in CRC patients. a-b Relative abundance of bacterial phyla (a) and genera (b) in fecal microbiota of healthy, BRAFV600E and BRAF wt subjects. c Heatmap of one-way hierarchical clustering of differentially represented species among the three cohorts (q-values < 0.10 from Mann-Whitney test). A dual-color code counts for species up- (red) and down-represented (blue), respectively. d Differences in the relative abundances of Hungateiclostridium saccincola (Hs), Prevotella enoeca (Pe), Sutterella megalospaeroides (Sum), Victivallales bacterium CCUG44730 (Vb), Prevotella dentalis (Pd) and Stenotrophomonas maltophila (Stm) (top) and Bacteroides dorei (Bd), Bacteroides ovatus (Bo), Lachnoclostridium phocaeense (Lp) and Ruthenibacterium lactatiformans (Rl) (bottom) markers in BRAF-mutated vs. BRAF wt CRC counterpart. P values from Mann-Whitney test are shown. e Differences in the relative abundance of predicted function for specific KEGG modules (level 2) in pairwise comparisons between healthy and BRAFV600E (top) or BRAF wt subjects (middle) and between BRAFV600E and BRAF wt CRC cases (bottom). q values are from Mann-Whitney test. f-g Receiver operating characteristic (ROC) curves for the single metagenomic classifiers Pe or Rl (f) and for the combination of the 10 markers Hs, Pe, Sum, Vb, Pd, Stm, Bd, Bo, Lp and Rl (g) in discriminating BRAF-mutated from BRAF wt CRC cases