Fig. 6
MIEF2 over-expression is mainly mediated by down-regulation of miR-424-5p in OC. a and b qRT-PCR and Western blot analyses for the expression of MIEF2 in OVCAR3 and ES2 cells after transfection with synthetic miR-424-5p. c Correlation between the expressions of MIEF2 and miR-424-5p in tumor tissues from 30 OC patients. d The expression of miR-424-5p was determined by qRT-PCR analysis in paired tumor and peritumor tissues from 30 OC patients. e The expression of MIEF2 was determined by Western blot analysis in SKOV3 and HEY cells transfected with indicated vectors. f and g MTS cell viability and colony formation assays in SKOV3 and HEY cells transfected with indicated vectors. h and i Wound-healing and matrigel invasion assays in SKOV3 and HEY cells transfected with indicated vectors. *P < 0.05