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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: MIEF2 over-expression promotes tumor growth and metastasis through reprogramming of glucose metabolism in ovarian cancer

Fig. 7

MIEF2 enhanced the Warburg effect in ovarian cancer cells. a Oxygen consumption rate (OCR) was determined in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. b Relative activities of respiratory complexes I, II, III, IV and V were determined in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. c ATP production was measured in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. d Representative transmission electron microscopy images of mitochondrial in OC cells with treatment as indicated. Scale bars, 0.2 μm. Mitochondrial length and cristae width were quantitatively analyzed. e and f Relative glucose consumption and lactate production were determined in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. g PH value in cell culture medium was determined in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. h Relative intracellular levels of intermediates in glycolysis and TCA cycle were determined by gas chromatography–time of flight–mass spectrometry (GC-MS) analysis in OVCAR3 and SKOV3 cells with MIEF2 knocked-down or over-expressed. *P < 0.05

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