Fig. 3

miRNA-30b-5p was identified to be a target of MINPP1 contributing to the development of HBV-positive HCC. a Alluvial diagram showing the association of 10 most significant pathways involved in HCC development with miRNAs and their predicted target genes. A: Signaling pathways regulating pluripotency of stem cells. b Associational of miRNAs and their predicted targeted genes with pathways. A: p53 signaling pathway. c Prediction of binding sites of miRNA-30b-5p on the MINPP1 transcript. d The interaction network of miRNA and mRNA. The red, blue, and yellow dot represent MINPP1, PGM2, and, LDHC, respectively, involved in glycolysis/gluconeogenesis pathway. The green dots represent differentially expressed miRNAs associated with three genes. e RT-qPCR results of MINPP1 expression level in Hep3B cells after treatment with inhibitor and mimics of miRNA-30b-5p. f Relative activity of luciferase reporters with MINPP1-UTR after co-transfection with miRNA-30b-5p mimics or mutant in Hep3B cells. ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001