Fig. 4

miRNA-30b-5p is an oncogene that regulates MINPP1. a RT-qPCR results of miRNA-30b-5p expression level in 20 paired HBV-positive and HBV-negative HCC tissues. b RT-qPCR results of miRNA-30b-5p expression level in hepatoma cell lines. c Cell proliferation was assessed with the CCK-8 assay in Hep3B cell in which miRNA-30b-5p was inhibited (left) and overexpressed (right). d Left: The tumor formation in nude mice injected subcutaneously with Hep3B cell in which miRNA-30b-5p was inhibited (right) and control (left). Right: The harvested xenograft tumors. e The tumor volume was measured after the nude mice were treated with Hep3B cell in which miRNA-30b-5p was inhibited and control. f Tumor weight was measured after the nude mice were treated with Hep3B cells transfected with the inhibitor of miRNA-30b-5p and control construct. g RNA FISH revealed the co-localization of miRNA-30b-5p and MINPP1 in the HBV-positive HCC sample. The miR-30b-5p was labeled with CY3 and MINPP1 was labeled with FAM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, NS: no statistics