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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells

Fig. 7

Anti-GRP78 blockades exogenous GRP78-derived reinforcement of MES phenotype. a Western blotting for FLAG tag and total GRP78 in GSC20 transduced with vector control or WT FLAG-GRP78. b Nonpermeabilized Immunofluorescence staining of FLAG (red) in GSC20 transfected with FLAG-GRP78, taking vector as control. Scale bar, 10 μm, and 25 μm for the large field of view. c Western blotting for MES-related signatures CD44, YKL40 and pathways STAT3, p-STAT3, p65, p-p65 and C/EBPβ in GSC20 transfected with FLAG-GRP78 or vector control. d Western blotting for CD44, p65, p-p65 and C/EBPβ in GSC20 expressing FLAG-GRP78 with or without anti-GRP78 cotreatment for 48 h. e Flowcytometry demonstrated the apoptosis of GSC20 with 3 Gy radiation, which transfected with vector or FLAG-GRP78, with or without anti-GRP78 cotreatment. f Summary diagram for the possible mechanisms of csGRP78 playing an essential role in maintaining MES phenotype and targeting csGRP78 impairs BACE2 stabilization via lysosome-dependent degradation in MES subtype GSCs. Error bar indicates at least three independent experiments and data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

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