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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Promotion of epithelial-mesenchymal transformation by hepatocellular carcinoma-educated macrophages through Wnt2b/β-catenin/c-Myc signaling and reprogramming glycolysis

Fig. 1

HCC-TCM induces M2 polarization of macrophages. THP-1 derived macrophages (THP-1-M) were incubated with 50% HCC-TCM for 48 h to obtain HCC-educated macrophages (HCC-TAMs). a The expression levels of CD163 on HCC-TAMs were detected by flow cytometry. b Transcript expression levels of markers for M1 or M2 macrophages were determined in HCC-TAMs by qPCR. HCC-TAMs were incubated with RPMI 1640 for an additional 24 h to obtain the condition medium (CM). c Huh-7 or SMMC-7721 cells were scratched with a plastic pipette tip and incubated with culture medium (Ctrl) or CM for 24 h. The results of this wound healing assay were photographed and measured. One representative of at least three independent experiments is shown. d Huh-7 or SMMC-7721 cells were incubated with the indicated volume of CM for 24 h. The cell viability of each group was detected by MTT assay. qPCR, quantitative real-time PCR; HCC, hepatocellular carcinoma; TCM, tumour condition culture medium; TAMs, tumour-associated macrophages; 7721, SMMC-7721. Data are presented as mean ± SEM from at least three independent experiments (*p < 0.05, **p < 0.01 and ***p < 0.001)

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