Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Promotion of epithelial-mesenchymal transformation by hepatocellular carcinoma-educated macrophages through Wnt2b/β-catenin/c-Myc signaling and reprogramming glycolysis

Fig. 5

CpG ODN blocks TCM-induced M2 polarization and downregulates the expression of Wnt2b/β-catenin in HCC-TAMs. THP-1 derived macrophages (THP-1-M) were treated with HCC-TCM for 48 h in the presence or absence of CpG ODN (2 μg/mL). a, b The expression levels of CD163 and markers for M1 or M2 macrophages on/in these TAMs were determined by flow cytometry and qPCR, respectively. c The expression levels of Wnt2b in these TAMs were determined by qPCR. d, e The expression levels of β-catenin in THP-1-M and indicated TAMs were determined by western blotting and immunofluorescence, respectively. f These TAMs were incubated with RPMI 1640 for another 24 h to obtain the condition medium (CM). HCC cells were cultured in the presence of indicated CM for 48 h. The expression levels of EMT markers were determined by western blotting. One representative of at least three independent experiments is shown. qPCR, quantitative real-time PCR; HCC, hepatocellular carcinoma; TCM, tumour condition culture medium; TAMs, tumour-associated macrophages; 7721, SMMC-7721. Data are presented as means ± SEM from at least three independent experiments (*p < 0.05, **p < 0.01)

Back to article page