Fig. 6

CpG ODN suppresses the glycolysis of HCC-TAMs via c-Myc. THP-1 derived macrophages (THP-1-M) were incubated with 50% HCC-TCM for 48 h in the presence or absence of CpG ODN (2 μg/mL). a The extracellular acidification rate (ECAR) of the indicated macrophages were measured with a seahorse analyser. b The mRNA expression levels of key enzymes involved in glycolysis were determined in Huh-7-TAMs by qPCR. c, d The mRNA expression levels of c-Myc were determined in Huh-7-TAMs by qPCR. qPCR, quantitative real-time PCR; HCC, hepatocellular carcinoma; TCM, tumour condition culture medium; 7721, SMMC-7721. Data are presented as mean ± SEM from at least two (a) or three independent experiments (b, c, d) (*p < 0.05, **p < 0.01)