Fig. 4

miR-375 regulates the proliferation and invasion of glioma through the CTGF-EGFR signalling pathway. a. Western blot analysis of CTGF, p-EGFR (Tyr1068), EGFR, p-AKT (Ser-473), AKT, and MMP9 in U87 and U251 cells. MiR-375 significantly down-regulates CTGF and its downstream molecules p-EGFR (Tyr1068), p-AKT (Ser-473), and MMP9. CTGF or EGF reversed the protein levels of p-EGFR (Tyr1068), p-AKT (Ser-473), and MMP9. b, c. Effect of CTGF or EGF on the growth inhibitory effect of miR-375, as determined by CCK-8 and EdU analysis. Scale bar = 100 μm. d. Wound healing analysis demonstrating the effect of CTGF or EGF on the inhibitory effect of miR-375 on glioma migration. Scale bar = 400 μm. e. Effect of CTGF or EGF addition on the inhibitory effect of miR-375 on glioma cell migration (left) and invasion (right) using Transwell analysis. Scale bar = 100 μm. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001. ns, not significant