Fig. 6

Exosomes regulate the proliferation and invasion of glioma through miR-375-CTGF-EGFR signalling pathway. a. Changes in miR-375 levels in miR-375 overexpressing U87 and U251 cells treated with or without GW4869 were examined by qRT-PCR. b-c. CCK-8 and EdU analysis of the effect elicited by GW4869 on the inhibitory effect of miR-375 on the proliferation of U87 and U251 cells. Scale bar = 100 μm. d. Wound healing analysis demonstrating the effect of GW4869 on the inhibitory effect of miR-375 on the migration of U87 and U251 cells. Scale bar = 400 μm. e. Transwell analysis showing the effect of GW4869 on the inhibitory effect of miR-375 on glioma cell migration (left) and invasion (right). Scale bar = 100 μm. f. Western blot analysis of CTGF, p-EGFR (Tyr1068), EGFR, p-AKT (Ser-473), AKT, and MMP9 in U87 and U251 cells. g. Effect of monensin on the inhibitory effect of miR-375 on CTGF and its downstream molecules p-EGFR (Tyr1068), p-AKT (Ser-473), and MMP9. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001. ns, not significant