Fig. 3

Depletion of TSP-4 in CAFs dampens CAFs-induced tumorgenicity of GBC-SD cells along with reversing the mesenchymal and stem-like phenotypes. a Representative images of subcutaneous xenografts in nude mice implanted with GBC-SD alone, GBC-SD + CAFs and GBC-SD + CAFs (shTSP-4) (n = 6). b, c Xenografts weight (mg) and tumor sizes were monitored and undergone quantification analysis. n = 6, **P < 0.01 by ANOVA for tumor weight; **P < 0.01 by repeated-measures ANOVA for tumor sizes. d, e Immunohistochemistry staining and semiquantitative analysis of Ki-67, E-cadherin, Vimentin, Sox2, Nanog and CD44 in xenograft tissues from different groups. Magnification is × 400, the scale bar represents 20 μm. n = 6, *P < 0.05 or **P < 0.01 by ANOVA