Skip to main content
Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Let-7a regulates EV secretion and mitochondrial oxidative phosphorylation by targeting SNAP23 in colorectal cancer

Fig. 2

Let-7a regulates EV secretion in CRC cells. a EVs released by different CRC cells were detected by electron microscopy. Scale bar, 100 nm. b Western blot analysis of indicated proteins in total cell lysate or EVs from CRC cells, with Calnexin as the negative controls for EVs. c qPCR analysis of let-7a expression in EVs derived from FHC cell and CRC cell lines. d Top 15 most abundant miRNAs identified in CRC cell-derived EVs from the Database: GSE125905. e qPCR analysis of let-7a expression in SW620 transfected with ctrl or let-7a mimic. f qPCR analysis of let-7a expression in SW480 transfected with ctrl or let-7a inhibitor. g Effect of let-7a mimic or inhibitor on EV secretion from CRC cells using Nanosight particle tracking analysis. h Effect of let-7a mimic or inhibitor on EV proteins from CRC cell-derived EVs. Data represent the mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001

Back to article page