Fig. 4

Let-7a/SNAP23 suppresses the proliferation of CRC cells. The proliferation of SW620 and SW480 CRC cells transfected with let-7a mimic or let-7a inhibitor was detected by CCK-8 (a) and EdU assays (b). Scale bar, 200 μM. The proliferation of CRC cells transfected with sh-SNAP23 or overexpressing SNAP23 was detected CCK-8 (c) and EdU assays (d). Scale bar, 200 μM. e Western blot analysis of cell cycle related proteins in SW620 and SW480 after the transfection of let-7a mimic or inhibitor and sh-SNAP23 or overexpressing SNAP23. f Cell proliferation by CCK-8 (left) and western blot of SNAP23 and Cyclin-D1 (right) in SW620 cells overexpressing vector and SNAP23 treated with ctrl or let-7a mimic. g Cell proliferation by CCK-8 (left) and western blot of indicated proteins (right) was shown in SW480 sh-NT and sh-SNAP23 treated with ctrl or let-7a inhibitor. Data represent the mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001