Fig. 1

CDK13 is upregulated in PCa tissues and the upregulation of CDK13 promoted proliferation in PCa cells. a, RT-qPCR detected CDK13 mRNA expression in 30 pairs of PCa and benign prostatic hyperplasia (BPH). *P < 0.05 vs. BPH. b, CDK13 protein expression was measured by Western blotting in 3 pairs of randomly selected BPH (B) and PCa (P) tissues. c, Hematoxylin and eosin (HE) staining of BPH and PCa tissues. d, Immunochemistry staining of CDK13 in BPH and PCa tissues. Scale bar = 50 mm. e, The expression levels of CDK13 mRNA in BPH and PCa tissues from the GSE13507 database (P = 0.0007). f, The expression of CDK13 was examined by RT-qPCR (up panel) or Western blotting (down panel) in human normal prostate epithelial cells (RWPE-1) and PCa cell lines (LNCaP, PC3, 22RV1 and DU145). CDK13 expression was significantly increased in PC3 and 22RV1 cell lines. *P < 0.05 vs. RWPE-1. g and h, Cell viability was measured by MTS assay (G) and colony formation assays (H) in PC3 and 22RV1 cell lines after transfected with indicated vectors. *P < 0.05, and **P < 0.01 vs. their respective empty vector. i, PC3 and 22RV1 cells were transfected with shCDK13 or control vector, cell apoptosis was detected by AnnexinV/PI flow cytometry. Right panel shows the apoptosis rate of three independent experiments. **P < 0.001 vs. pLKO. pWPI is a control vector of oeCDK13 and pLKO is the control vector of shCDK13