Fig. 5

(a) Wound closure determined by wound width (scratch assay; n = 15–16) and (b) migration velocity (time lapse microscopy after 24 h incubation; n = 46) of AT1 cells kept at pH 7.4 or. (c) Relative adhesion of AT1 cells up to 48 h. Cells were analyzed either during continuous direct incubation at pH 6.6 for 48 h (C1 direct incubation; n = 3–4) or after 24 h pre-incubation at pH 6.6 after which the medium was changed to pH 7.4 for the following 48 h (C2 pre-incubation; n = 6). (*) p < 0.05, (**) p < 0.01 vs. pH 7.4