Fig. 2

Exosomal ANGPTL1 attenuated liver metastasis and impeded vascular leakiness in the liver PMN. a Experimental scheme of exosome education and intrasplenic injection mouse model. b Liver metastasis was monitored by the IVIS Lumina imaging system. Represent images (left) and the fluorescence intensity (right; n = 3) of the mice livers at the fourth-week post tumor injection are shown. c H&E examination (left, arrows indicate tumors) and lesion areas (right; n = 5) of mice livers were used for metastatic burden assessment. Scale bar, 200 um. d In vivo vascular permeability measured by the appearance FITC-Dextran (green) injected by tail vein (left; n = 3). Data were presented as FITC-dextran /DAPI ratio (right). Scale bar, 20 um. Error bar indicates SEM of the mean. Data in panel b and d were analyzed using t-test, in panel c were analyzed using the Mann-Whitney test. *P < 0.05, **P < 0.01, ****P < 0.0001