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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Exosomal ANGPTL1 attenuates colorectal cancer liver metastasis by regulating Kupffer cell secretion pattern and impeding MMP9 induced vascular leakiness

Fig. 5

Exosomal ANGPTL1 decrease MMP9 production in KCs by inhibiting the JAK2-STAT3 signaling pathway. a KEGG pathway enrichment analysis of the changing genes between ImKC educated by Ctrl-Exo and ANGPTL1-Exo. The top 11 significant pathways were shown. b WB validation of the JAK2-STAT3 pathway in IMK educated by PBS, Ctrl-Exo and ANGPTL1-Exo at different times. c WB analysis of STAT3 phosphorylation in liver tissues from mice educated by PBS, Ctrl-Exo and ANGPTL1-Exo for 3 weeks (right). The number under the first band indicates the value of P-STAT3/STAT3 and the data were analyzed using t-test (left). d WB validation of STAT3 activation induced by IL-6 (left) and qRT-PCR analysis of MMP9 expression (right) in ImKC treated by ANGPTL1-Exo with or without IL-6 for 24 h. e Schematic diagram of the role of exosomal ANGPTL1 in CRC liver metastasis. Data are presented as the mean ± SEM of three independent experiments, and analyzed using t-test. *P < 0.05

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