Fig. 2

Genetic silencing of ACTN1 suppresses in vitro cell proliferation of HCC cells. a The mRNA expression level of ACTN1 in liver cancer cell lines, including HepG2, Huh-7, LM3, MHCC-97H, MHCC-97 L, SK-Hep1, SMMC-7721, SNU-423 and SNU-475 cells. b Real-time qPCR analysis of the knockdown efficiency of ACTN1 in MHCC-97H and SNU-423 cells. The experiments were repeated three times. c Western blotting analysis of the knockdown efficiency of ACTN1 in MHCC-97H and SNU-423 cells. d CCK8 assay of sh-ACTN1 and sh-Ctrl MHCC-97H and SNU-423 cell viability at 0, 24, 48 and 72 h time points. The experiments were repeated three times. e Colony formation assay of sh-ACTN1 and sh-Ctrl MHCC-97H and SNU-423 cell viability at 48 h time point. The experiments were repeated three times. *P < 0.05; **P < 0.01; ***P < 0.001