Fig. 4

ACTN1 regulates Hippo signaling activity and Rho GTPases activities. a The Cancer Genome Atlas (TCGA) RNA sequencing data of HCC cohort were downloaded and divided into two ACTN1-high and ACTN1-low groups. Gene set enrichment analysis (GSEA) was then preformed. False discovery rate (FDR) was set at 0.25. b Western blotting analysis of phospho-LATS1, LATS1, phospho-YAP and YAP in sh-ACTN1–1 and sh-Ctrl MHCC-97H cells. The activities of RhoA, Cdc42 and Rac1 were measured by pull-down assays in sh-ACTN1–1 and sh-Ctrl MHCC-97H cells. Statistical analyses of densitometry were shown at right. The experiments were repeated three times. c Real-time qPCR analysis of the mRNA levels of CTGF, ANKRD1 and CYR61 in sh-ACTN1–1 and sh-Ctrl MHCC-97H cells. The experiments were repeated three times. d Correlation analysis of ACTN1 expression with YAP target genes in the TCGA cohort. *P < 0.05; **P < 0.01