Fig. 2
From: A receptor-antibody hybrid hampering MET-driven metastatic spread
Binding analysis of the receptor-antibody hybrid proteins. A - Binding of the hybrids to HGF. DO24 anti-MET (100 ng/well) was used as capture antibody for purified recombinant fusion proteins (5 nM) in solid phase; increasing concentrations of HGF (0–10 nM) were added in liquid phase. The decoyMETK842E was used as control. B - Binding of the hybrids to decoyMET. Purified decoyMET (100 ng/well) was in solid phase, and increasing concentrations of the recombinant fusion proteins (0–1000 nM) were added in liquid phase. The MvDN30_long-sc60 was used as control. Kd values are reported below each graph. Each point is the mean of values in triplicate ± SD. Data reported in the figure are representative of one experiment out of two