Fig. 5

AbDec-L1 inhibits MET activation and invasion of CL-901 in vitro. A - Cytofluorimeter analysis of CL-901 cells stained with anti-MET antibodies. B -Immunoblotting analysis of HGF-induced MET phosphorylation in CL-901 cells treated with 1 μM AbDec-L1 and stimulated with 100 ng/ml HGF. Total cell lysates were immunoblotted with anti-phosphoMET (upper panel), anti-MET (middle panel) or anti-HSP90 antibodies (lower panel). C - Transwell invasion assay. CL-901 cells were stimulated with 25 ng/ml HGF and then treated with 1 μM AbDec-L1. Bars represent the percentage of invasion in comparison to the HGF-stimulated control. The figure panel below the graph shows one representative image/group of the cells migrated through the matrigel layer. Magnification, 25x. Each point is the mean of values in triplicate. Data reported in the figure are representative of one experiment out of three