Fig. 2

Pharmacological inhibition of HSF1 suppressed the initiation of pancreatic cancer. a Representative histopathology (H&E staining), low-grade PanINs lesions (AB/PAS staining) and the IHC staining of amylase (acinar marker), CK19 (ductal marker) in the pancreas of vehicle C mice, vehicle KC mice and KC mice treated with KRIBB11 (HSF1 inhibitor). b-f Quantification of the PanINs numbers (b) and the percentage of the PanINs area (c), the AB/PAS-positive blue area (d), the CK19-positive ductal area (e) and the amylase-positive acinar area (f) per 400× field in the pancreas of vehicle C mice, vehicle KC mice and KC mice treated with KRIBB11. g-h IHC staining and its quantification of Ki67-positive proliferative cells in PanINs structures per 400× field in the pancreas of vehicle KC mice and KC mice treated with KRIBB11. i Western blotting assay were performed to evaluate the effect of KRIBB11 on the expression of amylase, CK19, HSF1 and HSP70 and PCNA in KC mice pancreases. Scale bars = 25/50 μm. nd = not detected, * P < 0.05