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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: The EGFR-HSF1 axis accelerates the tumorigenesis of pancreatic cancer

Fig. 6

EGFR-HSF1 axis is momentous in pancreatic cancer initiation. a Representative histopathology (H&E staining), low-grade PanINs lesions (AB/PAS staining) and the IHC staining of amylase (acinar marker), CK19 (ductal marker), p-EGFR, HSP70 and Ki67 in the pancreas of vehicle KC mice and KC mice treated with erlotinib. b Quantification of the number of early and late PanINs per 400× field in the pancreas of vehicle KC mice and KC mice treated with erlotinib. c Quantification of the percentage of the precancerous lesion area, the AB/PAS-positive blue area, the amylase-positive acinar area, the CK19-positive ductal area, the p-EGFR-positive area and HSP70-positive area per 400× field in the pancreas of vehicle KC mice and KC mice treated with erlotinib. d Quantification of the number of HSF1- and Ki67-positive cells per 400× field in the pancreas of vehicle KC mice and KC mice treated with erlotinib. e Western blotting assay were performed to evaluate the effect of erlotinib on the expression of p-EGFR, EGFR, HSF1, HSP70 and PCNA in KC mice pancreas tissues. f Representative bright field/H&E staining images of 3D acinar/ductal-like spheres in the vehicle, EGF, EGF plus erlotinib (Erl) and EGF plus KRIBB11 (KRI) groups. g-h Quantification of the sphere number (g) and diameter (h) per 100× field in the vehicle, EGF, EGF plus erlotinib and EGF plus KRIBB11 groups (the EGF group was a control). i Western blotting was performed to evaluate the expression of acinar marker amylase, ductal marker CK19 and HSF1 target gene HSP70 among vehicle, EGF, EGF plus erlotinib, erlotinib, EGF plus KRIBB11 and KRIBB11 group in 3D acinar/ductal-like spheres. j Relative amylase, CK19 and HSP70 mRNA levels of 3D acinar/ductal-like spheres among the vehicle, EGF, EGF pluserlotinib and EGF plus KRIBB11 groups (the EGF group was a control). Scale bars = 50 μm. * P < 0.05

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