Fig. 3

LincSCRG1 negatively regulates miR26a by acting as a ceRNA. a The binding sites of miR26a on lincSCRG1are represented. b qPCR was performed to evaluate the expression of miR26a/b, 203b, 345 and 1297 in ov-lincSCRG1 Hep3B cells and sh-lincSCRG1 SNU-387 cells. c The expression of miR26a was examined in different cell lines (LO2, AML12, PHC, HepG2, Hep3B, HCCLM3, SNU-387) by qPCR. d Overexpression efficiencies of mi-miR26a and mi-NC were examined in the SNU-387 cell line, and interference efficiencies of in-miR26a and in-NC were examined in the Hep3B cell line by qPCR. RIP experiments (e) and luciferase reporter assays (f) were performed to demonstrate that miR26a was bound to lincSCRG1 in Hep3B and SNU-387 cells. g qPCR analysis showed that miR26a suppressed the expression of lincSCRG1, which could be promoted by in-miR26a. In b, ^***indicatesvs. The ov-vector/sh-NC group, p < 0.001; In (c), ^*/**/***indicatesvs. The LO2 group (^*, p < 0.05, ^**, p < 0.01, ^***, p < 0.001); In (d, f), ^***indicatesvs. The mi-NC/in-NC group, p < 0.001; In (e), ^***indicatesvs. The IgG group, p < 0.001; In (g) ^***indicatesmi-miR26a vs. the mi-NC group, p < 0.001; ^####indicatesin-miR26a vs. the in-NC group, p < 0.001