Fig. 4

MiR26a is negatively correlated with the proliferation and migration of HCC in vitro. Mi-miR26a, mi-NC (in SNU-387 cells), in-miR26a and in-NC (in Hep3B cells) cell lines were established. a Cell viabilitywas examined by MTT assays. b Oncogenic survival wasassessed by colony formation assays. c Cell cycle proliferation was evaluated by flow cytometry. d Migration was determined by transwell assays. e Cell cycle-related proteins (CKD4/6 and cyclinD1) and EMT-related proteins (MMP-2/3/9, E-cadherin, N-cadherin and Vimentin) were examined by western blot analysis. In (a-e), ^*/**/***indicatesvs. The mi-NC/in-NC group (^*, p < 0.05, ^**, p < 0.01, ^***, p < 0.001)