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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Linc-SCRG1 accelerates progression of hepatocellular carcinoma as a ceRNA of miR26a to derepress SKP2

Fig. 5

SKP2 is the direct target gene of miR26a in HCC in vitro. a qPCR was performed to evaluate the expression of SKP2, WNK1, MAT2A and EIF5A2 in Hep3B cells and SNU-387 cells. b SKP2 consensus binding site sequence and miR26a sequence alignment. The binding sequence is shown. c The SKP2 mRNA and protein expression was examined in SNU-387 cells and Hep3B cells treated with mi-miR26a. d Luciferase activity was strongly decreased when miR26a and SKP2-wt were cotransfected in SNU-387 cells and Hep3B cells in a luciferase reporter assay. e Sh-NC, sh-lincSCRG1, sh-lincSCRG1 + ov-SKP2 and sh-lincSCRG1 + in-miR26a groups were established in SNU-387 and Hep-3B cell lines. The expression of SKP2 mRNA and proteins wasstrongly impeded by sh-lincSCRG1, but this effect was reversed by in-miR26a and ov-SKP2 in SNU-387 cells and Hep3B cells. In (a-c) **/***indicates vs. the mi-NC/in-NC group (**, p < 0.01, ***, p < 0.001); In (d) ***indicatesvs. The mi-miR26a + SKP2-mut,p < 0.001. In (e) **indicates vs. the sh-NC group, p < 0.01; ##/###indicates vs. the sh-lincSCRG1 group (##, p < 0.01, ###, p < 0.01); &&indicatesvs. The sh-lincSCRG1 group, p < 0.01

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