Fig. 1

KDM4B is necessary for CRC cells proliferation and glucose metabolism. a Cell-cycle progression analysis was measured by propodium iodide staining and flow cytometry in LoVo cells transfected with siControl and siKDM4B 1#/2#. Representative histograms from an individual experiment and similar results were obtained in three independent experiments. b BrdUrd incorporation into DNA and DNA content in nuclei were determined by flow cytometry analysis in LoVo siControl and siKDM4B 1#/2# cells. c Intracellular glucose uptake was evaluated by 2-NBDG, a fluorescently tagged glucose derivative in KDM4B-depressed LoVo cells (siKDM4B 1#/2#) and KDM4B-overexpressed LoVo cells (KDM4B). d Intracellular ATP levels were measured by firefly luciferase-based bioluminescence ATP assay in KDM4B-depressed/overexpressed LoVo cells. e The expression of glucose-induced TXNIP was used to sense intracellular glucose uptake. Cells were incubated in glucose-free medium for 12 h, followed by glucose stimulation for an additional 3 h in LoVo cells and SW620 cells