Fig. 6

ABCB-1 inhibitor, elacridar, increases the efficacy of sunitinib. a Cell viability assay of sunitinib-sensitive Caki-1WT and the conditioned cell-line Caki-1 DC showing that ABCB-1 inhibition with elacridar significantly decreased cell viability of Caki-1 DC, which is comparable to Caki-1WT and b) Western blot showing slight increase of ABCB-1 protein level with sunitinib treatment in both Caki-1WT and Caki-1 DC, which did not change with elacridar. c Caki-1WT bearing embryos were treated with either vehicle, 10 μM SUT, 5 μM ELA or 10 μM SUT with 5 μM ELA combination treatment (left). The tumor size significantly decreased with SUT monotherapy and SUT with ELA combination treatment evaluated by optical image and optical coherence tomography (OCT) (bar-graph, right). d However, Caki-1 DC inoculated embryos responded only to 10 μM SUT and 5 μM ELA combination treatment and not to vehicle or monotherapies (bar-graph, right). SUT: sunitinib. ELA: elacridar. Data are mean ± SEM and normalised to matched controls, n = 3–5 independent experiments. Average of 3 to 5 CAM tumor bearing embryos. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001