Fig. 4
Combined treatment of Sora and Sim enhanced the sensitivity of LM3-SR cells to Sora. (a) The combined treatment analysis of Sora and Sim using Calcusyn. The dose-effect curve, Fa-CI plot and Fa-DRI plots are shown. Sora (15 μM) and Sim (10 μM) resulted in CI value of 0.722, and the DRI for Sora was 2.843. (b) Hoechst 33342 staining of Sora and Sim co-treatment in LM3-SR cells. (c) Flow cytometry analysis of the effect of Sora and Sim co-treatment in LM3-SR cells. (d) Glycolysis levels of Sora and Sim co-treatment in LM3-SR cells, reflected by lactate production and glucose uptake levels. (e) Western blotting analysis of critical proteins. (f) Effect of Sora and Sim co-treatment induced by LM3-SR cells using the xenograft tumor model. (g) H&E and TUNEL staining of tumor slices. (h) H&E staining of liver, kidney, lung and heart after Sim treatment (200 x). (i) Serum lipid levels, including TG, TCHO, LDL-C and HDL-C (n = 4). The data were represented as mean ± SD. * indicates p < 0.05 vs. CTRL group. # indicates p < 0.05 vs. Sora group