Table 2 Primers used for qPCR
Gene name | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
PKM2 | ATGTCGAAGCCCCATAGTGAA | TGGGTGGTGAATCAATGTCCA |
HK2 | GAGCCACCACTCACCCTACT | CCAGGCATTCGGCAATGTG |
PFKFB1 | AGAAGGGGCTCATCCATACCC | CTCTCGTCGATACTGGCCTAA |
PFKFB2 | TGGGCCTCCTACATGACCAA | CAGTTGAGGTAGCGTGTTAGTTT |
PFKFB3 | TTGGCGTCCCCACAAAAGT | AGTTGTAGGAGCTGTACTGCTT |
PFKFB4 | TCCCCACGGGAATTGACAC | GGGCACACCAATCCAGTTCA |
LDH-A | ATGGCAACTCTAAAGGATCAGC | CCAACCCCAACAACTGTAATCT |
LDH-B | TGGTATGGCGTGTGCTATCAG | TTGGCGGTCACAGAATAATCTTT |
LDH-C | AGAACATGGTGATTCTAGTGTGC | ACAGTCCAATAGCCCAAGAGG |
HIF-1α | GAACGTCGAAAAGAAAAGTCTCG | CCTTATCAAGATGCGAACTCACA |
AMPK-α1 | TTGAAACCTGAAAATGTCCTGCT | GGTGAGCCACAACTTGTTCTT |
AMPK-α2 | GTGAAGATCGGACACTACGTG | CTGCCACTTTATGGCCTGTTA |
AMPK-β1 | CCACTCCGAGGAAATCAAGGC | CTGGGCGGGAGCTTTATCA |
GLUT1 | GGCCAAGAGTGTGCTAAAGAA | ACAGCGTTGATGCCAGACAG |
β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
PGC1 | TCTGAGTCTGTATGGAGTGACAT | CCAAGTCGTTCACATCTAGTTCA |
PPRC1 | CAAGCGCCGTATGGGACTTT | GGAGGCATCCATGTAGCTCT |
PPAR-α | ATGGTGGACACGGAAAGCC | CGATGGATTGCGAAATCTCTTGG |
PPAR-γ | GGGATCAGCTCCGTGGATCT | TGCACTTTGGTACTCTTGAAGTT |