Fig. 4

Inhibition of miR-200b blocked the effects of PinX1overexpreesion on cell EMT, migration and invasion in nasopharyngeal CD133⁺ CSCs. a The mRNA expressions of E-cadherin, Vimentin, Snail1, Twist1, and Zeb1 in nasopharyngeal CD133⁺ CSCs transfected with empty vector and pcDNA3.0-PinX1 and pcDNA3.0-PinX1 + miR-200b inhibitor as determined by RT-qPCR. b The protein levels of E-cadherin, Vimentin, Snail1, Twist1, and Zeb1 in nasopharyngeal CD133⁺ CSCs transfected with empty vector and pcDNA3.0-PinX1 and pcDNA3.0-PinX1+ miR-200b inhibitor as determined by Western blot. The grey value of the protein was normalized to that of the corresponding GAPDH. c Cell migration and invasion following transfection as measured by Transwell assay. * p < 0.05 vs. control, ** p < 0.01 vs. control, *** p < 0.001 vs. control