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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Pin2 telomeric repeat factor 1-interacting telomerase inhibitor 1 (PinX1) inhibits nasopharyngeal cancer cell stemness: implication for cancer progression and therapeutic targeting

Fig. 6

Inhibition of miR-200b blocked the effects of PinX1 overexpression on cell proliferation and EMT of nasopharyngeal CD133+ CSCs in vivo. a The xenograft mice models bearing tumours originating from CD133+ CSCs, n = 5 /group. b tumour volume was periodically measured for each mice. c tumour growth curves was plotted. d The protein level of Key EMT-related markers was detected by western blot in xenografts derived from nasopharyngeal CD133+ CSCs after overexpression of PinX1 and co-inhibition of miR-200b. The grey value of the protein was normalized to that of the corresponding GAPDH. e The mRNA level of Key EMT-related markers was detected by RT-qPCR in xenografts derived from nasopharyngeal CD133+ CSCs after overexpression of PinX1 and co-inhibition of miR-200b. f Representative H&E staining of primary cancer tissues are shown as well as immunohistochemistry (IHC) detection of E-cadherin and Vimentin in xenografts derived from nasopharyngeal CD133+ CSCs. Magnification × 400. Scale bar: 30 μm. * p < 0.05 vs. control, ** p < 0.01 vs. control

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