Fig. 5

MYCN regulates endogenous expression of PLAGL2 in neuroblastoma cells. a-b Effect of MYCN knockdown on PLAGL2 mRNA and protein levels. Cells were transfected with siMYCN or siControl at 25 nM for 2 days. PLAGL2 mRNA (a) and protein (b) levels were detected as above. c Effect of MYCN overexpression on PLAGL2 expression in MYCN non-amplified neuroblastoma cells. Cells were transfected with a MYCN over-expression vector or a control vector for 2 days. The levels of N-Myc protein and PLAGL2 mRNA were detected as above. *, p < 0.05, comparing to control. Protein levels in the Western blot images were quantified as above