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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: The PLAGL2/MYCN/miR-506-3p interplay regulates neuroblastoma cell fate and associates with neuroblastoma progression

Fig. 7

PLAGL2 regulates neuroblastoma cell survival, proliferation and differentiation. a Effect of siPLAG2 and siMYCN on viability of BE(2)-C cells. Cells were transfected with the indicated siRNAs or control oligo (25 nM) for 4 days, and cell viability was measured. Dashed line indicates the predicated additive effect of combined siPLAGL2 and siMYCN treatment. *, p < 0.05 comparing to control; **, p < 0.05 comparing to siPLAGL2 or siMYCN single treatment. b Western blots of PLAGL2 and N-Myc proteins under the same treatments as above confirmed the depletion of the protein expressions by siRNAs. c Time-course of cell confluence change of BE(2)-C cells after siPLAGL2 transfection. Cells were transfected with 25 nM siPLAGL2 or siControl, and cell confluence was measured using IncuCyte Zoom. *, p < 0.05, comparing to siControl at the same time point. d Effect of PLAGL2 knockdown on colony formation of BE(2)-C cells. Cells were transfected with 25 nM siPLAGL2 or siControl, and 2000 transfected cells were seeded on 100 mm dishes and cultured for 2 weeks. Crystal violet was used to stain the colonies. Show are the representative images of the dishes. *, p < 0.05. e Effect of siPLAGL2 on the protein expression levels of cell differentiation, cell proliferation and apoptosis markers. Cells were transfected with the indicated oligo and protein levels were detected and quantified as above. f-g Effect of PLAGL2 knockdown on neurite outgrowth in BE(2)-C cells. Cells were transfected with 25 nM oligos for 4 days, and relative neurite lengths were quantified. Shown are representative cell images analyzed to define neurite and cell body areas (f) and neurite length quantifications (g)

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