Fig. 2

c-Jun is crucial for the enhanced PD-L1 expression in drug-resistant A549/CDDP, MCF7/ADR and HepG2/ADR cells. Drug-resistant A549/CDDP (a and b), MCF7/ADR (c and d) and HepG2/ADR (e and f) cells were transfected with c-Jun-targeting siRNAs for 24 h (for qRT-PCR) or 48 h (for western blotting), and PD-L1 expression was detected by qRT-PCR or western blotting. Parental A549 (g and h), MCF7 (i and j) and HepG2 (k and l) cells were transfected with c-Jun expression plasmids for 24 h (for qRT-PCR) or 48 h (for western blotting), and PD-L1 expression was detected by qRT-PCR or western blotting. All experiments were performed independently in triplicate. ^*P ≤ 0.05, ^**P ≤ 0.01, ^***P ≤ 0.001