Fig. 4

FKBP9-M541I mutant displays a weaker carcinogenesis compared with the wide type counterpart. a Efficiencies for stable overexpresssion of FKBP9 in SF-539, T98G and U-87 MG cells of FKBP9 (indicated as PCDH-vector, FKBP9-WT and FKBP9-M541I) were tested by IB assay. b, c Analysis of the ability of FKBP9-WT- and FKBP9-M541I-overexpressed SF-539, T98G and/or U-87 MG cells to form colonies and spheres. Expression of Nestin, Sox2 and/or Oct4 were detected by IB. d Images of mice tumors from SF-539-PCDH-vector, SF-539-FKBP9 and SF-539-M541I cells 45 days post injection. (n = 5 mice for each). Tumor volumes were measured. e HE staining and IHC analysis of FKBP9 and Ki67 expression were performed. Scale bar = 50 μm. (*p < 0.05, **p < 0.01, ***p < 0.001)