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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: YAP1 plays a key role of the conversion of normal fibroblasts into cancer-associated fibroblasts that contribute to prostate cancer progression

Fig. 2

YAP1 plays an important role in the conversion of NFs to CAFs in vitro. a-b The mRNA expression of YAP1 and α-SMA in the CAF, CAFshYAP1, NF and NFoverexpressYAP1 groups were detected by qRT-PCR. c The protein expression of YAP1, FAP and α-SMA in the indicated four cell lines were detected by western blot. GAPDH was used as an endogenous reference gene. d Immunofluorescence staining shows the expression level and location of YAP1 and α-SMA in the four indicated four cells. The nuclei were stained with DAPI. The representative image had a magnification of 400 x. e-f The MTT experiment showing the effect of the conditioned medium on the four indicated cell lines on the proliferation of the epithelial cells TrampC1 or RM1. The absorbance value was detected at a wavelength of 570 nm (*P < 0.05). g The Transwell invasion assay detects the effect of the conditioned medium on the indicated four cell lines on the invasive ability of the epithelial cells TrampC1 or RM1. Statistical results (right side) of the above invasive ability. Five visual field counts were taken for each group, and the ordinate indicates the number of invading cells (***P < 0.001). h The protein expression of E-cad, N-cad and vimentin in the indicated four cell lines were detected by western blot. GAPDH was used as an endogenous reference gene

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