Fig. 5

Expression of YAP1 in the CAFs of prostate cancer patients. a Immunofluorescence staining for the detection of YAP1 and SRC expression in stromal cells of PCa samples (n = 25). The representative images had a magnification of 400 x. b Correlation between expression of YAP1 and SRC in prostate cancer stromal cells. The abscissa represents the positive rate of YAP1 in prostate cancer stromal cells, and the ordinate represents the positive rate of SRC in prostate cancer stromal cells. Pearson r = 0.7534, P < 0.001. c Primary cell culture was performed by clinical surgical specimens, and the primary hCAF and hNF cells were photographed under a light microscope. The representative images were magnified 200 x. d Immunofluorescence technique was used to detect the expression level and location of α-SMA in hNF and hCAF. The representative images were magnified 400 x. e The interaction between YAP1 and SRC in the hCAF was determined by the co-IP assay. The relative levels of YAP1 or SRC in these cells were determined by western blot using a YAP1 or SRC antibody. f Western blot was used to detect the protein expression levels of YAP1, SRC, FAP and α-SMA in hNF and hCAF. g The protein expression levels of YAP1, SRC and α-SMA were detected after transfection of hCAF with siYAP1. h The protein expression levels of YAP1 and α-SMA were detected after transfection of hCAF with siSRC. GAPDH was used as an endogenous reference gene. i The MTT experiment showing the effect of the conditioned medium on hCAF cell lines on the proliferation of the epithelial cells LNCaP or PC3. The absorbance value was detected at a wavelength of 570 nm (*P < 0.05). j The Transwell invasion assay detects the effect of the conditioned medium on hCAF cell lines on the invasive ability of the epithelial cells LNCaP or PC3. Statistical results (right side) of the above invasive ability. Five visual field counts were taken for each group, and the ordinate indicates the number of invading cells (***P < 0.001)