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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: TMEM52B suppression promotes cancer cell survival and invasion through modulating E-cadherin stability and EGFR activity

Fig. 6

TMEM52B overexpression suppresses cancer cell invasion and survival and reduces phosphorylation of EGFR, MAPKs, and AKT. (A-C) SW480 cells were transfected with a TMEM52B-expression vector for 48 h. Invasion (A) and cell survival (B) assays were performed as described in Fig. 2A and B, respectively. (C) Transfected cells were lysed for immunoblot analysis. Anti-myc was used to detect myc-tagged TMEM52B. (D) SW480 cells were co-transfected with a TMEM52B-expression vector and an AP-1 reporter plasmid for 48 h. AP-1 activity was determined by a reporter assay as described in Materials and Methods. (E, F) HEK293E cells were co-transfected with an EGFR-expression vector and a TMEM52B-expression vector for 48 h. (E) Transfected cells (2 × 104 cells) were allowed to invade Matrigel for 48 h in the absence or presence of EGF (10 ng/ml). Cell invasion was determined by calculating the cell-stained area relative to the total area using ImageJ software. All determinations were performed in three independent experiments. Values represent mean ± SD. *P < 0.05. (F) Transfected cells were lysed for immunoblot analysis. An anti-myc antibody was used to detect myc-tagged TMEM52B

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