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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Hsa_circ_0005273 facilitates breast cancer tumorigenesis by regulating YAP1-hippo signaling pathway

Fig. 1

The characteristic of hsa_circ_0005273 in BC cells. A Hsa_circ_0005273 is formed by circularization of exon 27–29 of the gene PTK2, and the splicing junction was verified by Sanger sequencing. B Existence of hsa_circ_0005273 in BC cells was verified by agarose gel electrophoresis. C Expression of hsa_circ_0005273 in BC cells with random hexamer or oligo (dT)18 primers. D-F RT-qPCR analysis of hsa_circ_0005273, linear PTK2 and β-actin in BC cells treated with RNase R. G-I Expression levels of cytoplasmic control transcripts (GAPDH), the nuclear control transcript (U6), and hsa_circ_0005273 were determined by RT-qPCR in the cytoplasmic and nuclear fractions of BC cells. J RNA FISH for hsa_circ_0005273 and nuclei was stained with DAPI. Red, hsa_circ_0005273; Blue, DAPI. *p < 0.05, **p < 0.01,*** p < 0.001,**** p < 0.0001

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